General Cell Based Assays
Bioassay Systems EnzyFluo™ NADP/NADPH Assay Kit. For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism (This product is a replacement for the discontinued product EFNP-100).
For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism (This product is a replacement for the discontinued product EFNP-100). Key Features: Sensitive and accurate. Detection limit of 0.01 µM and linearity up to 1 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Cell, tissue extracts etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.01 µM.
Eurofins Discoverx PathHunter eXpress AVPR1B Activated GPCR Internalization Assay, 10pl Kit. (93-0722E3CP5L)
The PathHunter AVPR1B Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of AVPR1B via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged AVPR1B. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting AVPR1B-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mRXFP3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0928E2MCP2L)
The PathHunter mRXFP3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of mRXFP3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mRXFP3 and EA-tagged β-Arrestin-2. Activation of mRXFP3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress OPRL1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0115E2CP2M)
The cAMP Hunter OPRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orphanin FQ) based activation of OPRL1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRL1, a naturally Gi/Go coupled GPCR. Upon activation of OPRL1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human Tenascin C ASY
The R-PLEX Human Tenascin-C Assay from Meso Scale Discovery is an electrochemiluminescent immunoassay designed for the quantitative measurement of Tenascin-C in human serum plasma and cell culture supernatants This assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and rapid detection of Tenascin-C an extracellular matrix glycoprotein implicated in tissue injury and cancer progression With a detection range of 1 56-400 ng/mL and minimal required sample volume this assay allows high-throughput and accurate quantification of Tenascin-C levels from small sample sizes
Sigma Aldrich Fine Chemicals Biosciences L Lactate Assay Kit suffic
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is therefore a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple direct and automation-ready procedures for measuring lactate concentration are very desirable.
Cayman Chemical Cell-Based Assay Buffer Tablet, 5 ea
Cell-Based Assay Buffer Tablet has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Eurofins Discoverx PathHunter GLP2R Bioassay Kit, 10-Plate. (93-0572Y2-00134)
The PathHunter GLP2R Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. GLP2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress OPRM1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0213E3CP0L)
The PathHunter OPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. met 5-enkephalin) based activation of OPRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRM1 and EA-tagged β-Arrestin-2. Activation of OPRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR2C (VGV) U2OS β-Arrestin GPCR Assay, 10pl Kit. (93-0932E3CP19L)
The PathHunter HTR2C(VGV) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C(VGV) GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C(VGV) and EA-tagged β-Arrestin-2. Activation of HTR2C(VGV) stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems EnzyChrom™ Aspartate Transaminase Assay Kit. For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity.
For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity. Key Features: Sensitive. Linear detection range: 2-100 U/L. Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 2 U/L.
Apexbio Technology LLC JC-10 Mitochondrial Membrane Potential Assay Kit(Synonyms: JC-10 Assay Kit, JC-10 MMP Assay Kit, JC-10 mitochondrial dye assay), 100T.
The JC-10 Mitochondrial Membrane Potential Assay Kit utilizes the fluorescent probe JC-10 to assess mitochondrial membrane potential ( m) a critical marker for mitochondrial function and early apoptosis detection JC-10 selectively accumulates in mitochondria with intact membrane potential forming red-fluorescent aggregates Upon loss of m associated with apoptosis or mitochondrial dysfunction JC-10 disperses into monomeric forms emitting green fluorescence The mitochondrial membrane potential is therefore indicated by changes in the ratio between red and green fluorescence intensity This assay is suitable for studies involving cultured cells isolated mitochondria or tissue samples and is commonly employed in apoptosis research and mitochondrial functional analysis
Cayman Chemical Urea Fluorometric Assay Kit, 96 Well
Urea is the final degradation product of protein and amino acid metabolism. Urea is found in blood and is excreted by the kidney as a component of urine. Besides its role as carrier of waste nitrogen, urea also plays a role in the countercurrent exchange system of the nephrons, allowing for re-absorption of water and critical ions from the excreted urine. Cayman’s Urea Fluorometric Assay provides a convenient method for detecting urea in plasma, serum, and urine. In this assay, urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Ammonia reacts with the detector resulting in a fluorescent product. Fluorescence is analyzed with an excitation wavelength of 405-415 nm and an emission wavelength of 470-480 nm.
Eurofins Discoverx PathHunter Detection Kit, 550 (100 plates), 40,000 dp (384-well). (93-0001L)
The PathHunter Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter cell lines or with custom cell lines made using ProLabel/PK expression vectors. This kit contains lysis buffer and EFC substrate to allow for detection of EFC levels in the assayed cells. This kit contains all components required to assay 100 plates (96/384 well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0001l.